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handle: 10261/34193
[ES] El sistema comprende una construcción de ADN que comprende: a) una primera secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para un producto de interés; b) una segunda secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para un dominio de dimerización; y c) una tercera secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para la u-hemolisina (HlyA) de Escherichia coli o para un fragmento de dicha proteína que comprende la señal de reconocimiento del mecanismo de secreción del sistema transportador de hemolisina (Hly) de E. Coli. De aplicación en la producción de proteínas recombinantes diméricas.
[EN] The system comprises a DNA construction comprising: a) a first sequence of nucleic acid containing the sequence of nucleotides coding for a product of interest; b) a second sequence of nucleic acid containing the sequence of nucleotides coding for a dimerization domain and c) a third sequence of nucleic acid containing the sequence of nucleotides coding for a-hemolysin (HlyA) of Escherichia coli or for a fragment of said protein comprising the signal recognizing the secretion mechanism of the transport system of hemolysin (Hly) of E. Coli. The method can be applied in the production of recombinant dimeric proteins.
Fecha de presentación internacional: 19.07.2004. - Titular: Consejo Superior de Investigaciones Científicas (CSIC)
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