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System for the production of dimeric proteins based on the transport system of hemolysin of escherichia coli

Authors: Lorenzo, Víctor de; Fernández, Luis Ángel;

System for the production of dimeric proteins based on the transport system of hemolysin of escherichia coli

Abstract

[ES] El sistema comprende una construcción de ADN que comprende: a) una primera secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para un producto de interés; b) una segunda secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para un dominio de dimerización; y c) una tercera secuencia de ácido nucleico que contiene la secuencia de nucleótidos que codifica para la u-hemolisina (HlyA) de Escherichia coli o para un fragmento de dicha proteína que comprende la señal de reconocimiento del mecanismo de secreción del sistema transportador de hemolisina (Hly) de E. Coli. De aplicación en la producción de proteínas recombinantes diméricas.

[EN] The system comprises a DNA construction comprising: a) a first sequence of nucleic acid containing the sequence of nucleotides coding for a product of interest; b) a second sequence of nucleic acid containing the sequence of nucleotides coding for a dimerization domain and c) a third sequence of nucleic acid containing the sequence of nucleotides coding for a-hemolysin (HlyA) of Escherichia coli or for a fragment of said protein comprising the signal recognizing the secretion mechanism of the transport system of hemolysin (Hly) of E. Coli. The method can be applied in the production of recombinant dimeric proteins.

Fecha de presentación internacional: 19.07.2004. - Titular: Consejo Superior de Investigaciones Científicas (CSIC)

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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