Microplastics (MPs) are acknowledged as emerging contaminants that pose a substantial threat to the environment. The adverse impacts of MP pollution extend across marine, freshwater, and terrestrial ecosystems, covering regions from the Tropics to the Poles. Although our comprehension of MP behavior has progressed in recent years, it is still difficult to predict exposure hotspots or exposure scenarios. Despite a noteworthy increase in data concerning MP occurrence in different environmental compartments and species, there is a noticeable scarcity of experimental data on MP uptake, accumulation, and effects. This Virtual Special Issue (VSI) received a total of 19 contributions from 11 countries, with a significant majority originating from Italy, India, Spain, and China. These contributions were categorized into three main themes: the occurrence and effects of MPs on aquatic and terrestrial organisms, the presence of chemical additives in plastics, and review articles summarizing previously published research on MPs.