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Autolytic yeasts (BGS4 DELTA), production method thereof and applications of same in protein expression methods

Authors: Ribas, Juan Carlos; Durán, Ángel; Cortés, Juan Carlos G.;

Autolytic yeasts (BGS4 DELTA), production method thereof and applications of same in protein expression methods

Abstract

[EN] The invention relates to novel yeasts which are effective for the expression and production of commercial heterologous proteins, enabling the secretion thereof to the medium by means of controlled autolysis. The invention is advantageous in that (i) natural lysis takes place, without causing any other type of cellular alterations as happens in other methods, and (ii) cell breakage is induced, thereby preventing any physical treatment method that would otherwise be necessary in order to break the cell wall of the yeast and release the contents thereof. The invention also relates to the method of producing said yeasts and to the applications thereof.

[ES] La invención describe unas nuevas levaduras eficaces para la expresión y obtención de proteínas heterólogas de interés comercial que permite su secreción al medio mediante autolisis regulada. La ventaja es que se produce una lisis natural, sin producir otro tipo de alteraciones celulares como ocurre en otros procedimientos y que la rotura celular es inducida, por lo que se evita cualquier método de tratamiento físico, de otra manera necesario para romper la pared celular de la levadura y liberar su contenido. Igualmente, se describe su método de obtención y sus aplicaciones.

Fecha de presentación internacional: 25.04.2005. - Titular: Consejo Superior de Investigaciones Científicas (CSIC)

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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OpenAIRE UsageCountsViews provided by UsageCounts
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