Chromatographic behaviour and purification of N1.5-glycosyl enkephalins

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1988 Spain English Publisher:Springer Science and Business Media LLCJournal:Chromatographia, volume 25, pages 891-894 (issn: 0009-5893, eissn: 1612-1112,

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Authors: Torres, Josep Lluís; Clapés Saborit, Pere; Haro Villar, Isabel; Valencia Parera, Gregorio; Reig Isart, Francisca; García-Antón, José María;

doi: 10.1007/bf02311424

handle: 10261/331744

Chromatographic behaviour and purification of N1.5-glycosyl enkephalins

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Abstract

N1.5-Glycosyl enkephalins are potent analgesics. Two enkephalin-related glycosyl peptides: [DMet2, Pro5] enkephalin (N1.5 β-glucopyranosyl] amide and [DMet2, Pro5] enkephalin [N1.5 β-galactosyl] amide have been prepared following the liquid phase procedure. The chromatographic behavior of both the final glycosyl enkephalins and their synthetic intermediates on reversed-phase columns has been tested. Semipreparative, reversed-phase, HPLC conditions have been developed in order to obtain a simple and rapid procedure for the purification of the two synthetic pentapeptides. Thus, using isocratic elution, injections of 10mg of crude material after gel filtration gave homogeneous (99.9%) glycosyl enkephalins. © 1988 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

This work has been supported by the Spanish Comision Asesora de Investigación Científica y Técnica (CAICYT).

Peer reviewed

Country

Spain

Related Organizations

Spanish National Research Council
Spain

Keywords

Glycosylenkephalins, Opioids, Analgesic peptides, Glycopeptides, Column liquid chromatography

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