Estimation of the effective orientation of the SHG source in primary cortical neurons

descriptionPublicationkeyboard_double_arrow_right Article 31 Jul 2009 English Publisher:Optica Publishing GroupJournal:Optics Express, volume 17, page 14,418 (eissn: 1094-4087,

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Authors: Psilodimitrakopoulos, Sotiris; Petegnief, Valérie; Soria, Guadalupe; Amat-Roldan, Ivan; Artigas, David; Planas, Anna M.; Loza-Álvarez, Pablo;

doi: 10.1364/oe.17.014418

pmid: 19654849

handle: 10261/31606

Estimation of the effective orientation of the SHG source in primary cortical neurons

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Abstract

In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at thetae =33.96 degrees , with a bandwidth of Deltathetae = 12.85 degrees . This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules.

Related Organizations

Spanish National Research Council
Spain
Institute of Biomedical Research of Barcelona
Spain
Institute of Photonic Sciences
Spain

Keywords

Cerebral Cortex, Neurons, Diagnostic applications of nonlinear optics, Light, Scanning microscopy, Polarimetric imaging, Optical Devices, Equipment Design, Models, Theoretical, Harmonic generation and mixing, Image Enhancement, Nonlinear microscopy, Rats, Equipment Failure Analysis, Rats, Sprague-Dawley, Tissue characterization, Microscopy, Fluorescence, Medical and biological imaging, Animals, Computer-Aided Design, Scattering, Radiation, Computer Simulation

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