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Desarrollo y evaluación de microsatélites derivados de EST para la gestión ecuménica de la merluza europea Merluccius merluccius

Authors: Santafé-Muñoz, A. (Angie); Pérez, M. (Montse); Iglesias-Estévez, J. (José); Sánchez-Conde, F.J. (Francisco Javier); Pita, A. (Alfonso); Presa, P. (Pablo);

Desarrollo y evaluación de microsatélites derivados de EST para la gestión ecuménica de la merluza europea Merluccius merluccius

Abstract

El gran valor comercial y organoléptico de la merluza europea (Merluccius merluccius) junto con su sobreexplotación en las últimas décadas,la convierten en una candidata potencial en acuicultura marina. Partiendo de 476.747 secuencias de una genoteca del transcriptoma de merluza europea, se caracterizaron 200 microsatélites, de los cuales 1610ci (2 tetranucleótidos, 8 trinucleótidos y 6 dinucleótidos), se combinaron en peA· múltiplex junto con cinco marcadores previos para el genotipado sistemático de poblaciones y stocks domésticos de merluza (lEO-Vigo). Su aplicación a 192 merluzas salvajes representativas de su rango natural muestran que este conjunto de microsatélttes derivados de EST son una herramienta genética potente, tanto analítica como estadisticamente. Además, la homologia de la secuencia de algunos clones con genes expresados en otras especies, puede pemnír la selección asistida por marcadores de genes candidatos, además del manejo reproductivo del stock de merluza.

The high commercial and organoleptic value 01 the European hake (Merluccius merluccius) together with its overexploitation during lhe last decades, make this species a promising candidate to marine aquaculture.ln this study we have screened 476,747 sequences Irom a transcriptome library 01 the European hake. Sixteen microsatellite loci (2 tetranucleotides, 8 trinucleotides, 6 dinucleotides) out 01two·hundred ones tested were selecled and multiplexed with live previous markers lorthe systematic genotyping 01 populations and hatchery hake stocks (IEO·Vigo). Multiplexes testing on 192 wild hakes showed that this EST-derived microsatellite set is analytically and statistically powerful regarding its polymorphism and would allow marker assisted seleclion olcandidate genes in this species.

Country
Spain
Keywords

Acuicultura, Centro Oceanográfico de Vigo

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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