This study aimed to determine the maximum emersion time of Solea senegalensis specimens, as well as stress level caused by this procedure. Results will be useful for its application in transport. For this purpose, 56 Senegalese soles of 922.33 ± 29.52 g were individually placed in polystyrene boxes on a wet cloth, maintaining constant temperature during emersion (15 °C). Specimens (n = 7) were sampled for plasma at different points (0, 2, 6, 12, 20, 28, 36, and 44 hours of emersion), and several stress and metabolic parameters were assessed. After that, each experimental group was placed in recovery tanks for assessing delayed mortality after emersion trial. The results showed that a waterless transport is possible without direct or delayed mortality up to 28 hours. From this moment, high stress level indicated by i) significantly cortisol and lactate enhancement, as well as ii) glucose reserves mobilization originated physiological problems during emersion that induced mortality of specimens.