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DIGITAL.CSIC
Master thesis . 2023 . Peer-reviewed
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Nutrición y fisiología de las paralarvas del pulpo común Octopus vulgaris

Authors: Mokhtar, S.;

Nutrición y fisiología de las paralarvas del pulpo común Octopus vulgaris

Abstract

La alta mortalidad registrada durante el desarrollo larvario debida en parte a la ausencia de una dieta adecuada obstaculiza el cultivo comercial de Octopus vulgaris. La comprensión de la fisiología digestiva constituye una de las líneas de investigacion. En este estudio intentamos mejorar el conocimiento sobre la alimentación, nutrición y fisiología de paralarvas de O. vulgaris mediante el diseño de una dieta inerte y el estudio del efecto de diferentes dietas sobre la capacidad enzimática digestiva de las paralarvas y su viabilidad. En un primer ensayo se analizó la actividad enzimática digestiva de paralarvas alimentadas con zoeas de Maja brachydactyla y gammáridos, encontrándose una mayor actividad especifica en ejemplares de 10 días independientemente de la dieta. Adicionalmente se llevaron a cabo dos ensayos de cultivo donde se probó el efecto de alimentar a las paralarvas por un lado con zoeas de Grapsus adscensionis y por otro con Artemia enriquecida con la microalga Navícula salinicola y fosfolípidos marinos. Desafortunadamente, en ambos casos los cultivos se tuvieron que cerrar antes de tiempo por la aparición de una contaminación, no encontrándose diferencias entre los tratamientos. Finalmente se diseñó una dieta inerte en base a la composición bioquímica de ejemplares silvestres obtenida de la bibliografía. Con esta información se seleccionaron las materias primas y se usó la técnica de esferificación directa con alginato para la fabricación de la microdieta. Se llevó acabo un cultivo comparando el efecto de esta dieta, tanto fresca como liofilizada en coalimentación con Artemia frente a un tratamiento control, los datos mostraron un mayor crecimiento al alimentar con ambas dietas y una mayor actividad de las proteasas alcalinas, especialmente al usar dieta fresca.

The high mortality recorded during larval development due in part to the absence of an adequate diet hampers the commercial cultivation of Octopus vulgaris. The understanding of the digestive physiology constitutes one of the lines of research. In this study we try to improve the knowledge about the feeding, nutrition and physiology of O. vulgaris paralarvae through the design of an inert diet and the study of the effect of different diets on the digestive enzymatic capacity of the paralarvae and their viability. In a first study, the digestive enzymatic activity of paralarvae fed with Maja Brachydactyla zoeas and gammarids was analysed, finding a higher specific activity in 10-day old specimens regardless of diet. Additionally, two culture trials were carried out where the effect of feeding the paralarvae on the one hand with zoeas of Grapsus adscensionis and on the other with Artemia enriched with the microalgae Navicula salinicola and marine phospholipids was tested. Unfortunately, in both cases the cultures had to be closed prematurely due to the appearance of contamination, not finding differences between the treatments. Finally, an inert diet was designed based on the biochemical composition of wild specimens obtained from the bibliography. With this information the raw materials were selected and the direct spherification technique with alginate was used to manufacture the micro diet. A culture was carried out comparing the effect of this diet, both fresh and lyophilized in co-feeding with Artemia against a control treatment, the data showed greater growth when feeding with both diets and a greater activity of alkaline proteases, especially when using fresh diet.

Máster en Cultivos Marinos ; 2019-2020. Diploma de Máster of Science en Acuicultura otorgado por el Centro Internacional de Altos Estudios Agronómicos del Mediterráneo en Zaragoza (CIHEAM). Realizado en el COC-IEO Tenerife y ULPGC.

Country
Spain
Keywords

fish, marine invertebrates, Acuicultura, Physiology, Feeding, en, aquaculture statistics, Centro Oceanográfico de Canarias, Octopus vulgaris, Diets, 251092 Acuicultura marina, Nutrition

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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