Use of enzymatic cDNA amplification as a method of detection of bean yellow mosaic virus

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1993 Spain English Publisher:Springer Science and Business Media LLCJournal:Netherlands Journal of Plant Pathology, volume 99, pages 97-100 (issn: 0028-2944, eissn: 1573-8469,

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Authors: Castro, Serafina; Carazo, G.; Sáiz, Margarita; Romero, Javier; De Blas, C.;

doi: 10.1007/bf01998477

handle: 10261/295165

Use of enzymatic cDNA amplification as a method of detection of bean yellow mosaic virus

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Abstract

The C-terminal region of bean yellow mosaic virus (BYMV) coat protein gene was selected for the design of oligonucleotide primers. Reverse transcription of viral RNA present in sap of virus-infected plants followed by polymerase chain reaction (RT-PCR) was performed for amplification of a 449 baise pairs fragment. These primers supported BYMV amplification, but did not support amplification on both bean common mosaic virus (BCMV) and potato virus Y (PVY) templates. © 1993 Koninklijke Nederlandse Planteziektenkundige Vereniging.

Country

Spain

Related Organizations

Spanish National Research Council
Spain

Keywords

Detection, RT-PCR, Bean common mosaic virus, Potato virus Y

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