Interspecific relationship studies between A, S and D genome diploid species, and between AAGG and AABB allotetraploid species of the genus Triticum were conducted using isoenzymatic characters located in dry mature seeds. Data was analyzed by the factorial analysis of correspondences, and dendograms were obtained by two different genetic distances. The discussion of results was based on the limitations of the study, intraspecific variability differences, isoperoxidase frequency differences and chromosomal location of peroxidases in T. aestivum cv. 'Chinese Spring'. The closest relationship was found between 'dicoccoides' and 'carthlicum'. Relationships found between T. turgidum L. and T. timopheevvi Zhuk., both allotetraploid species and 'boeoticum'; this species and speltoides; tauschi and searsii, and the last two species with 'bicorne', are discussed at the phylogenetic level.