Four Spanish Isolates of Pear Decline Phytoplasma are Related to Other European Phytoplasmas of the Apple Proliferation Group

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 2001 Spain English Publisher:WileyJournal:Journal of Phytopathology, volume 149, pages 481-484 (issn: 0931-1785, eissn: 1439-0434,

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Authors: Martin, Robert R.; Carazo, G.; Arribas, Claudia; Colino, I.; Santiago, Remedios; De Blas, C.;

doi: 10.1046/j.1439-0434.2001.00657.x , 10.1111/j.1439-0434.2001.tb03881.x

handle: 10261/293945

Four Spanish Isolates of Pear Decline Phytoplasma are Related to Other European Phytoplasmas of the Apple Proliferation Group

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Abstract

AbstractA portion of about 1000 bp within the 16S rDNA of four pear decline (PD) symptomatic trees (two from Badajoz and two from Sevilla) was amplified by nested polymerase chain reaction (PCR) and directly sequenced. A sequence similarity search against the EMBL and GenBank nucleotide databases showed that they were most similar to two previously sequenced isolates of pear decline phytoplasma of German and Italian origin. In a phylogenetic analysis they clustered in a separate branch within the apple proliferation group. The four Spanish PD sequences were fully homologous to previously published primers R16F2n, primer forward (Ahrens and Seemüller. Phytopathology 82:828‐832, 1992) and PD probe. However, primers fPD, fU5 and R16F2 showed some mismatches with these sequences.

Country

Spain

Related Organizations

Spanish National Research Council
Spain

Keywords

Phytoplasma, Direct sequencing, Pear decline, 16S rRNA, Primer specifity, Polymerase chain reaction

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