PCR detection of sequences similar to the AS‐48 structural gene in bacteriocin‐producing enterococci

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 Spain English Publisher:Oxford University Press (OUP)Journal:Letters in Applied Microbiology, volume 24, pages 40-42 (issn: 0266-8254, eissn: 1472-765X,

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Authors: Joosten, H. M. L. J.; Rodríguez, Enrique; Núñez Gutiérrez, Manuel;

doi: 10.1046/j.1472-765x.1997.00349.x

pmid: 9024003

handle: 10261/292534

PCR detection of sequences similar to the AS‐48 structural gene in bacteriocin‐producing enterococci

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Abstract

Three oligonucleotide primers, specific for the structural gene of antimicrobial peptide AS-48, were used to study the distribution of sequences similar to this gene in 15 independently isolated bacteriocin-producing Enterococcus strains by semi-nested PCR. Eight of 10 Ent. faecalis strains and three of five Ent. faecium strains gave a positive result in both reactions of the semi-nested PCR. These findings indicate that bacteriocins produced by many enterococcal strains are very closely related or even identical to peptide AS-48.

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Spain

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Keywords

Bacteriocins, Genes, Bacterial, Sequence Homology, Nucleic Acid, Enterococcus faecium, Enterococcus faecalis, Peptides, Polymerase Chain Reaction, DNA Primers

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