A comparative study of the Gene-Trak Listeria assay, the Listeria-Tek ELISA test and the FDA method for the detection of Listeria species in raw milk

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1993 English Publisher:Oxford University Press (OUP)Journal:Letters in Applied Microbiology, volume 17, pages 178-181 (issn: 0266-8254, eissn: 1472-765X,

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Authors: Rodríguez, J. L.; Gaya, P.; Medina, M.; Nuñez, M.;

doi: 10.1111/j.1472-765x.1993.tb00389.x

handle: 10261/290553

A comparative study of the Gene-Trak Listeria assay, the Listeria-Tek ELISA test and the FDA method for the detection of Listeria species in raw milk

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Abstract

A total of 110 raw milk samples were analysed using the Gene‐Trak Listeria assay, unmodified and with four modifications to its enrichment procedure, the Listeria‐Tek ELISA test and the FDA method. Thirty‐five samples yielded confirmed positive results by at least one of the methods tested. The original Gene‐Trak protocol yielded 24 confirmed positive results, significantly higher than the 13 obtained with the Listeria‐Tek ELISA test or with the FDA method. The modifications to the Gene‐Trak Listeria assay did not significantly increase the detection rate of Listeria spp. Copyright © 1993, Wiley Blackwell. All rights reserved

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