A computer program for the design of PCR primers for diagnosis of highly variable genomes

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1993 English Publisher:Elsevier BVJournal:Journal of Virological Methods, volume 41, pages 157-165 (issn: 0166-0934,

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Authors: Dopazo, Joaquín; Sobrino Castelló, Francisco;

doi: 10.1016/0166-0934(93)90123-9

pmid: 8388397

handle: 10261/289992

A computer program for the design of PCR primers for diagnosis of highly variable genomes

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Abstract

PCRDiag (Diagnosis by PCR) is a computer program which allows the localization of pairs of oligonucleotides with optimal thermodynamic requirements for use in a PCR assay. The program is designed for the selection of pairs of primers complementary to sequences present in a group, whose identification is intended, but are absent in other non-specific sequences. The program constitutes a powerful tool, specially in systems which display a high degree of sequence heterogeneity, as is the case of RNA viruses. The program runs on IBM-PC and compatible computers and has no special software requirements. It does not need the previous alignment of the sequences analyzed.

Keywords

Genome, Base Sequence, Foot-and-mouth disease virus, Molecular Sequence Data, DNA, Single-Stranded, Genetic Variation, Polymerase Chain Reaction, PCR, Aphthovirus, Diagnosis, DNA, Viral, Computer-Aided Design, Thermodynamics, Computer software, Variability, Primer selection, Algorithms, Software

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