Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2003 English Publisher:Elsevier BVJournal:Veterinary Immunology and Immunopathology, volume 94, pages 77-81 (issn: 0165-2427,

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Authors: Domenech, N.; Rodríguez-Carreño, M. P.; Filgueira, P.; Alvarez, B.; Chamorro, S.; Domínguez, J.;

doi: 10.1016/s0165-2427(03)00084-9

pmid: 12842613

handle: 10261/289927

Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues

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Abstract

Here we report the successful use of monoclonal antibodies (mAbs) BL1H7, BA1C11 (anti-SWC3) and 4E9 for immunohistochemical identification of macrophages in formalin-fixed, paraffin-embedded porcine tissues. Retrieval of antigen reactivity was achieved by heating the slides in a domestic pressure cooker, which makes the technique suitable for the routine laboratory. This method allows to perform retrospective studies in routinely processed tissues and may be useful to investigate the role of macrophages in different pathological processes.

Keywords

Monoclonal antibody, Swine Diseases, Pig, Paraffin Embedding, Swine, Macrophages, Antibodies, Monoclonal, Immunohistochemistry, Sensitivity and Specificity, Paraffin, Formaldehyde, Animals, Lymph Nodes, Spleen, Retrospective Studies

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