In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 2009 English Publisher:Elsevier BVJournal:Vaccine, volume 27, pages 5,603-5,611 (issn: 0264-410X,

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Authors: Díaz, I.; Pujols, J.; Ganges, L.; Gimeno, M.; Darwich, L.; Domingo, M.; Mateu, E.;

doi: 10.1016/j.vaccine.2009.07.029

pmid: 19646408

handle: 10261/289743

In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus

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Abstract

T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-gamma ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic.

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Keywords

Bioinformatics, Swine, T-cell epitopes, Computational Biology, Epitopes, T-Lymphocyte, Nucleocapsid Proteins, Vaccines, Attenuated, Interferon-gamma, Viral Envelope Proteins, Porcine reproductive and respiratory syndrome virus, Leukocytes, Mononuclear, Vaccines, DNA, Animals, Porcine respiratory and reproductive syndrome virus

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