A New Method for Total Mercury and Methyl Mercury Analysis in Muscle of Seawater Fish

descriptionPublicationkeyboard_double_arrow_right Article 17 Apr 2009 English Publisher:Springer Science and Business Media LLCJournal:Bulletin of Environmental Contamination and Toxicology, volume 83, pages 210-213 (issn: 0007-4861, eissn: 1432-0800,

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Authors: Carbonell, G.; Bravo, J. C.; Fernández, Carlos; Tarazona, José V.;

doi: 10.1007/s00128-009-9720-x

pmid: 19373425

handle: 10261/289700

A New Method for Total Mercury and Methyl Mercury Analysis in Muscle of Seawater Fish

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Abstract

In this work we have developed a cost-effective method for the analysis of methyl mercury (MeHg) in seawater fish muscle. The novelty of this method lies in the use of microwave-assisted extraction with acidic solution (HCl), addition of toluene, and subsequent extraction with cysteine acetate solution where only MeHg is present because of its affinity for cysteine groups. MeHg in cysteine phase and total mercury in the homogenate muscle tissue were determined using a direct Hg analyzer (DMA-80). Validation, precision, and accuracy of the method were evaluated and monitored with a tuna fish certified reference material (CRM 463) containing MeHg.

Keywords

Tuna, Muscles, Methyl mercury, Mercury, Methylmercury Compounds, Microwave extraction, Direct mercury analyzer, Animals, Seawater, Microwaves, Water Pollutants, Chemical, Environmental Monitoring

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