Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2
Copyright policy )Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2
SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we present a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the non-replicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.
DNA vaccine, 2412 Inmunología, COVID-19 Vaccines, mouse model, ADN, Immunology, Inmunología, furina, N protein, Mouse model, 615.371, S protein, Tecnología farmaceútica, Mice, pPAL, 615.46, Vaccines, DNA, Animals, Humans, 3209 Farmacolog?a, 578.834, Pandemics, Furin, Mammals, Mice, Inbred BALB C, SARS-CoV-2, virus del SRAS, COVID-19, Viral Vaccines, DNA, 546, RC581-607, SARS Virus, Química inorgánica (Farmacia), Anti-Bacterial Agents, Mice, Inbred C57BL, 3209 Farmacología, Immunologic diseases. Allergy, 6310.03 Enfermedad, furin
DNA vaccine, 2412 Inmunología, COVID-19 Vaccines, mouse model, ADN, Immunology, Inmunología, furina, N protein, Mouse model, 615.371, S protein, Tecnología farmaceútica, Mice, pPAL, 615.46, Vaccines, DNA, Animals, Humans, 3209 Farmacolog?a, 578.834, Pandemics, Furin, Mammals, Mice, Inbred BALB C, SARS-CoV-2, virus del SRAS, COVID-19, Viral Vaccines, DNA, 546, RC581-607, SARS Virus, Química inorgánica (Farmacia), Anti-Bacterial Agents, Mice, Inbred C57BL, 3209 Farmacología, Immunologic diseases. Allergy, 6310.03 Enfermedad, furin
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