
handle: 10261/276608
[FR] Dans cette étude, un ensemble de 8 marqueurs microsatellites (SSRs) a été utilisé pour l´analyse de 84 individus appartenant à la banque de germoplasme de “Boughrara”- Sfax. De plus une observation morphologique des endocarpes des fruits a été réalisée afin de confirmer toutes les conclusions obtenues par l´analyse moléculaire. Le contenu en information polymorphique des marqueurs utilisés démontrent leur utilité dans l´identification et la classification des variétés d´olives tunisiennes. Les moyennes d´hétérozigoties et le nombre total d´allèles détectés attestent de la grande variabilité présente dans la collection en question. Cependant, de nombreuses duplications ont été détectées dans cette collection. L´analyse factorielle a permit d´indiquer les tendances d´agroupement entre les génotypes identifiés suivant leurs origines géographiques et l´usage de leurs fruits.
[EN] In this work, 84 accessions belonging to “Boughrara”- Sfax germoplasm bank have been screened using a set of 8 selected microsatellite markers (SSRs). Moreover, morphological observation of fruit endocarps has been also performed in order to confirm all conclusions made by the molecular screening. Averages of the polymorphic information content demonstrated that the majority of the utilised markers are very useful for identification and classification of Tunisian olive cultivars. A high variability within the collection was shown by the elevated averages of heterozygosity indexes and the total number of alleles. However, several duplications were detected within the collection. Factorial analysis displayed the cluster tendencies among the identified genotypes using geographical origin and fruit uses as the criteria.
Trabajo presentado al Olivebioteq 2009: For a renovated, profitable and competitive Mediterranean olive growing sector, celebrado en Sfax (Túnez) del 15 al 19 de diciembre de 2009.
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