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Evaluation of different methods to determine pollen quality in several olive (Olea europaea L.) cultivars

Authors: Rejón, Juan David; Suárez, Cynthia; Alché Ramírez, Juan de Dios; Castro López, Antonio Jesús; Rodríguez García, María I.;

Evaluation of different methods to determine pollen quality in several olive (Olea europaea L.) cultivars

Abstract

[ES] La calidad del polen es un parámetro de gran utilidad en estudio agro-biológico y que incluye tanto la viabilidad como la capacidad de germinación del grano de polen. En el caso del polen del olivo (Olea europaea L.) tiene especial inrerés para seleccionar cultivares polinizadores. En este trabajo se han analizado los siguientes métodos para evaluar la calidad del polen: a) unción con azul tripán, b) reacción fluorocromática con diacetato de fluoreceína y e) capacidad de germinación in v itro del polen. Los resultados obtenidos indican que los dos último son lo métodos más efectivos para la estimación de la calidad del polen, y han permitido clasificar los cultivares estudiados de acuerdo con su carácter de polinizador potencial.

[EN] Pollen quality is a useful parameter for agrobiological studies, which includes both the viability and rhe capacity of the pollen grain 10 germinate. In the case of the olive (Olea europaea L.) pollen, such parameter is of particular interest for the seleclion of pollen donar cultivar. In this work we have compared several method to evaluate the quality of pollen, including: a) trypan blue staining, b) the fluorochromatic reaction of the fluorescein diacetate, and e) the germtnation ability of pollen in vitro. The results obtained indicate that the two latter methods were the most effective to estimate olive pollen quality, and allowed us to classify the cultivars studied according to their pollinating potential.

Este trabajo ha sido financiado por los proyectos AGL2008-00517/AGR y BFU 2008-00629 (Ministerio de Ciencia e Innovación) y por la Consejería de Ciencia y Tecnología de la Junta de Andalucía (Grupo de Investigación BI0283). J. D. Rejón agradece al Ministerio de Ciencia e Innovación la concesión de una beca/contrato FPU.

Peer reviewed

Keywords

Calidad del polen, Azul tripán, Olea europaea, Pollen viability

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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