CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 2022 Spain, Spain English Publisher:Elsevier BVJournal:Developmental & Comparative Immunology, volume 133, page 104,431 (issn: 0145-305X,

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Authors: Belén Álvarez; Concepción Revilla; Sara Moreno; Ángeles Jiménez-Marín; Elena Ramos; Paloma Martínez de la Riva; Teresa Poderoso; +3 Authors

doi: 10.1016/j.dci.2022.104431

pmid: 35526640

handle: 10261/270603 , 10668/22152

CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells

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Abstract

In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4+ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4+ T cell development. CD4+ 2E3+ CD8α- CD9- cells are the least differentiated population of naïve cells, whereas the CD4+ 2E3- CD8α+CD9+ and CD4+ 2E3- CD8α+ CD9- cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9+ phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4+ T cell heterogeneity in swine.

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Spain, Spain

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Keywords

CD4-Positive T-Lymphocytes, Antígenos comunes de leucocito, Subgrupos de linfocitos T, Swine, CD4 T cells, Cell Differentiation, CD9, Memoria inmunológica, Porcinos, Linfocitos T CD4-positivos, Phenotype, Memory, T-Lymphocyte Subsets, Animals, Leukocyte Common Antigens, Diferenciación celular, Fenotipo, IFN-γ, Immunologic Memory, CCR7

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