
handle: 10261/27048
[ES] Se describe un método analítico, sencillo y rápido, para la cuantificación de escualeno en aceites comestibles. El método consiste en realizar primero una metilación alcalina en frío de la muestra grasa disuelta en hexano y, posterior análisis de la disolución de hexano por cromatografía gaseosa usando patrón interno. Se presentan una recta de calibrado con patrones cromatográficos puros, así como, los coeficientes de variación de muestras de aceites con alto, medio y bajo contenido en escualeno.
[EN] A simple and rapid analytical method for the quantification of squalene in vegetable oils is described. The method consists of a cold alkaline methylation of the oil sample diluted with hexane followed by quantitation by gas chromatography with internal standard. A calibration curve obtained using different standard solutions, and coefficients of variation for three different samples with high, medium and low content of squalene, are reported.
3 páginas, 3 figuras, 1 tabla.
Peer reviewed
Gas chromatography, Vegetable oil, Aceite vegetal, Escualeno (determinación), Cromatografía gaseosa, Squalene (determination)
Gas chromatography, Vegetable oil, Aceite vegetal, Escualeno (determinación), Cromatografía gaseosa, Squalene (determination)
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