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Mantenimiento de dos cepas patógenas de Vibrio anguillarum y V. splendidus en el rotífero Brachionus plicatilis

Authors: Prol García, María Jesús; Pintado, José;

Mantenimiento de dos cepas patógenas de Vibrio anguillarum y V. splendidus en el rotífero Brachionus plicatilis

Abstract

[ES] El objetivo de este trabajo fue estudiar el mantenimiento de dos cepas patógenas de peces, V. anguillarum 90-11-287 y V. splendidus DMC-1, en el rotífero B. plicatilis. La incorporación de los patógenos se llevó a cabo mediante la incubación del rotífero, previamente enriquecido con la microalga I. galbana, con los patógenos durante 3 h. El tiempo de permanencia de estas cepas en el rotífero y en el agua se estudio mediante técnicas de cultivo convencionales así como con técnicas moleculares (DGGE, PCR a tiempo real). Los resultados obtenidos revelaron con ambas bacterias una permanencia de 48 h en los rotíferos y 24 h en el agua, siendo este periodo de tiempo suficiente para causar un brote infeccioso en los cultivos larvarios. Además, la introducción de estas bacterias en los rotíferos parece causar modificaciones estables a largo plazo en la microbiota propia de estos organismos

[EN] The objective of this work was to study the maintenance of two fish pathogenic strains V. anguillarum 90-11-287 and V. splendidus DMC-1 in the rotifer B. plicatilis. The incorporation of the pathogens was carried out by the incubation of rotifers, previously enriched with the microalgae I. galbana, with the pathogens during 3 h. The time of permanence of those bacteria, in the rotifer and in water, was studied by culture methods as well as by molecular techniques (DGGE, real time PCR). The results obtained revealed a permanence of 48 h in the rotifer and 24 h in the water for both bacteria, being that period of time enough to cause a disease outbreak in the larval cultures. Furthermore, the introduction of those bacteria in the rotifer seems to cause variations, long-term stable, in the microbiota present in those organisms

Este trabajo se ha realizado en el marco del proyecto ACU003-03 financiado por el INIA. M. J. Prol disfruta de una beca predoctoral I3P del CSIC

4 pages, 2 figures.-- XI Congreso Nacional de Acuicultura, Vigo 25-28 de septiembre de 2007

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
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