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handle: 10261/265340
[ES] Hasta el momento, tanto las secuencias nucleotídicas y proteicas inmuno relevantes para la dorada (Sparus aurata) no están bien representadas en las bases de datos existentes. La razón es porque los proyectos de EST (Expressed Sequence Tag) que intentan identificar genes inmunes relacionados son realizados a partir de la secuenciación de librerías de cDNA de células inmunes y tejidos no estimulados. La regulación de los factores inmunes está bajo un control estricto y su expresión sólo es regulada cuando las células inmunes son estimuladas con PAMPs (Patrones Moleculares Asociados a Patógenos). Para poder obtener un incremento en el número de secuencias inmuno relevantes en dorada se construyó una librería de cDNA a partir de múltiples células y tejidos inmunes estimulados con diferentes PAMPs
[EN] To date, immune-relevant nucleotide and protein sequences for the seabream (Sparus aurata) are not well represented in currently available sequence databases. The most likely reason is because expressed sequence tag (EST) projects that set out to identify immune-related genes are developed by sequencing cDNA libraries from non-stimulated immune cells or tissues. Regulation of immune factors is under a strict control and their expression is only regulated when immune cells are stimulated by PAMPs (Pathogen Associated Molecular Patterns). In order to increase the number of immune-relevant sequences in seabream, a cDNA library was constructed with several immune cells and tissues stimulated with different PAMPs
4 pages, 2 figures, 1 table.-- XI Congreso Nacional de Acuicultura, Vigo 25-28 de septiembre de 2007
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