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Procedimiento de inmovilización de una glutamato deshidrogenasa

Authors: Bolívar Bolívar, Juan Manuel; Mateo González, César; Rocha-Martín, Javier; Fernández-Lafuente, Roberto; Guisán, José Manuel; Cava Valenciano, Felipe;

Procedimiento de inmovilización de una glutamato deshidrogenasa

Abstract

Procedimiento de inmovilización de una glutamato deshidrogenasa. Esta solicitud describe la preparación de un derivado de una glutamato deshidrogenasa (GDH) de Thermus spp inmovilizado-estabilizado por unión covalente multipuntual. La enzima soluble ya es muy estable desde pH 6 a pH 10, incluso a 60 C, reconoce NAD, NADH, NADP, y NADPH. Su inmovilización en soportes glioxil permite la estabilización tanto de su estructura multimérica como de la rigidez de cada monómero, aumentando aún más su termoresistencia, y ampliando el rango de utilización a pH inferiores a 5 (donde la enzima soluble se inactiva por disociación). El derivado inmovilizado es resistente a disolventes, alta temperatura, pHs drásticos, etc.

Fecha de solicitud: 17/09/2009.- Titular: Consejo Superior de Investigaciones Científicas (CSIC)

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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