Skeletal development and mineralization are essential processes driven by the coordinated action of neural signals, circulating molecules and local factors. The PTH family of peptides consists of at least six genes and plays a crucial role in the process of bone mineralization in fsh by regulating calciumphosphate homeostasis. The Pth4 peptide, recently isolated and characterized in zebrafsh, has been shown to be involved in bone metabolism through the regulation of phosphate in adult zebrafsh. By creating a transgenic Pth4:eNTR zebrafsh line we performed a nitroreductase-mediated cell ablation of the Pth4:eNTR-expressing neurons in zebrafsh larvae. This resulted in impaired mineralization and altered expression of phosphate regulation and osteoblast differentiation genes. To specifcally characterize the single-Pth4 cells transcriptional landscape, we devised a workflow, including tissue disaggregation, sorting of single cell populations by fluorescence activated cell sorting (FACS), total RNA isolation/purifcation and construction of libraries followed by high-throughput sequencing (CNAG-CRG). Bioinformatics analyses are underway and results will be presented at the conference

This work was funded by the Spanish Economy and Competitiveness Ministry project AGL2017- 89648P to L. Guerrero-Peña was supported by pre-doctoral fellowship FPI funded by Spanish Economy and Competitiveness Ministry (AGL2017-89648P ) and by pre-doctoral fellowship of the Spanish Personnel Research Training Program funded by Spanish Economy and Competitiveness Ministry (PRE2018-085475). L. Méndez-Martínez was supported by pre-doctoral fellowship of the Xunta De Galicia (IN606A-2020/006).

Trabajo presentado en el XIII Congress of AIEC online, celebrado en modalidad virtual del 16 al 17 de septiembre de 2021.

Peer reviewed