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Aislamiento y purificación de la vitelogenina de lubina (Dicentrarchus labrax L.)

Authors: Zanuy, Silvia; Carrillo, Manuel; Prat, Francisco; Copeland, P.; Covens, M.;

Aislamiento y purificación de la vitelogenina de lubina (Dicentrarchus labrax L.)

Abstract

[EN] An specific serum protein (vittellogenin) was identified and purified from plasma of 17-~-Estradiol treated inmature males. The protein was purified as follows: plasma was dialized, dialisis was followed by a cromatographic separation in 6 B Sepharosa and Concavaline A Sepharosa. The purified protein had similar electrophoretic pattern of a female-specific protein (vitellogenin). An antibody was prepared agains,the purified protein and its specificity demonstrated through inmunospotting. The similarity of this protein with sea bass vitellogenin is discussed as well as the possibilities of its plasma leveIs determination through an especific R.I.A.

[ES] La vitelogénesis o síntesis del vitelo, es una fase importante en la gametogénesis de las hembras de los teleósteos. Durante la vitelógenesis exógena se sintetiza la vitelogenina (proteina presursora de las pro~einas del vitelo) en el hígado y es transportada hacia los oocitos a través de la sangre. Dentro del estudio de la fisiología de la reproducción es de gran importancia la determinaciÓn de los niveles plasmáticos de esta proteina, ya que su detección nos permite concretar el periodo vitelogénico y, a su vez, en aquellas especies que no poseen dimorfismo sexual, hacer una diferencia clara ent~e machos y hembras

Resumen del trabajo presentado en el II Congreso Nacional de Acuicultura, celebrado en Santiago de Compostela (España), del 1 al 4 de diciembre de 1987

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Spain
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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