Abstract Neighbour-stranger discrimination is an important mechanism in social behaviour, as it allows the individual to avoid the high energetic costs of repeated agonistic interactions with neighbours. In some anuran species, the owners of territories respond more aggressively towards strangers than neighbours. Here, we investigated whether gladiator frog, Boana goiana , males discriminate between neighbours and strangers. This is a lek-breeding species; therefore, it does not defend fixed territories which are associated with a specific resource. We conducted a playback field experiment with a repeated-measures design, based on three treatments: (i) neighbour’s call in initial position, (ii) stranger’s call in initial position, and (iii) neighbour’s call in new position. We did not observe any systematic modification in the acoustic properties (call duration, dominant frequency, repetition rate) of the advertisement and short aggressive calls of B. goiana males given in response to our playback treatments. Our results indicate that B. goiana males do not exhibit vocally mediated ‘dear enemy’ relationships. We discuss the possible ecological and social factors that may have impeded the evolution of ‘dear enemy’ relationships in this species.