Downloads provided by UsageCountsAntimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus
Copyright policy ) Cristina Velázquez-Suárez; Rubén Cebrián; Carmen Gasca-Capote; Antonio Sorlózano-Puerto; José Gutiérrez-Fernández; Manuel Martínez-Bueno; Mercedes Maqueda; +1 Authors
Cristina Velázquez-Suárez; Rubén Cebrián; Carmen Gasca-Capote; Antonio Sorlózano-Puerto; José Gutiérrez-Fernández; Manuel Martínez-Bueno; Mercedes Maqueda; Eva Valdivia;
Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus
The treatment and hospital-spread-control of methicillin-resistant Staphylococcus aureus (MRSA) is an important challenge since these bacteria are involved in a considerable number of nosocomial infections that are difficult to treat and produce prolonged hospitalization, thus also increasing the risk of death. In fact, MRSA strains are frequently resistant to all β-lactam antibiotics, and co-resistances with other drugs such as macrolides, aminoglycosides, and lincosamides are usually reported, limiting the therapeutical options. To this must be added that the ability of these bacteria to form biofilms on hospital surfaces and devices confer high antibiotic resistance and favors horizontal gene transfer of genetic-resistant mobile elements, the spreading of infections, and relapses. Here, we genotypically and phenotypically characterized 100 clinically isolated S. aureus for their resistance to 18 antibiotics (33% of them were OXA resistant MRSA) and ability to form biofilms. From them, we selected 48 strains on the basis on genotype group, antimicrobial-resistance profile, and existing OXA resistance to be assayed against bacteriocin AS-48. The results showed that AS-48 was active against all strains, regardless of their clinical source, genotype, antimicrobial resistance profile, or biofilm formation capacity, and this activity was enhanced in the presence of the antimicrobial peptide lysozyme. Finally, we explored the effect of AS-48 on formed S. aureus biofilms, observing a reduction in S. aureus S-33 viability. Changes in the matrix structure of the biofilms as well as in the cell division process were observed with scanning electron microscopy in both S-33 and S-48 S. aureus strains.
Netherlands, Spain
enterocin AS-48, antibiotic resistance, Antibiotic resistance, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Patient Care::Hospitalization, Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycosides::Aminoglycosides, RM1-950, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Bacterial Proteins::Bacteriocins, Article, Staphylococcus aureus MRSA, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Recombination, Genetic::Gene Transfer, Horizontal, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Glycoside Hydrolases::Muramidase, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell Division, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Penicillins::Methicillin, Medical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial, Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Elementary Particles::Electrons, <i>Staphylococcus aureus</i> MRSA, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Biofilms, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Lactones::Macrolides, Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Pyrrolidines::Lincosamides, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Recurrence, Biofilms, Therapeutics. Pharmacology, biofilms, Medical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus::Methicillin-Resistant Staphylococcus aureus, Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Infection::Cross Infection, Enterocin AS-48
enterocin AS-48, antibiotic resistance, Antibiotic resistance, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Patient Care::Hospitalization, Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycosides::Aminoglycosides, RM1-950, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Bacterial Proteins::Bacteriocins, Article, Staphylococcus aureus MRSA, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Recombination, Genetic::Gene Transfer, Horizontal, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Glycoside Hydrolases::Muramidase, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell Division, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Penicillins::Methicillin, Medical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial, Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Elementary Particles::Electrons, <i>Staphylococcus aureus</i> MRSA, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Biofilms, Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Lactones::Macrolides, Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Pyrrolidines::Lincosamides, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Recurrence, Biofilms, Therapeutics. Pharmacology, biofilms, Medical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus::Methicillin-Resistant Staphylococcus aureus, Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Infection::Cross Infection, Enterocin AS-48
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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