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handle: 10261/245895 , 20.500.14243/69013
[ES]: Durante 1997 se observaron en cultivos de tomate {Lycopersicon esculentum) de Málaga y Almería, síntomas anómalos en hojas que se caracterizaban por amarilleo internervial, manchas color púrpura, rojizas o necróticas, enrollado de las hojas viejas y aspecto quebradizo de las mismas; identificándose en el año 2000, por primera vez en España, al Tomato chlorosis virus (ToCV) como agente causal de esta enfermedad. En años posteriores se empezaron a observar síntomas similares en cultivos de tomate de Sevilla, Murcia, Alicante, Castellón, Mallorca, Tenerife y Gran Canaria, identificándose también al ToCV como el agente causal en la mayoría de muestras analizadas. En verano de 2001 se identificó, en cultivos de tomate de la provincia de Castellón, la presencia de un nuevo virus en España: Tomato infectious chlorosis virus (TICV); que un año más tarde fue detectado en Alicante. ToCV y TICV son crinivirus trasmitidos por mosca blanca de manera semipersistente, limitados al floema y que infectan a tomate. ToCV es transmitido por Trialeurodes vaporariorum (Westwood), T. abutilonea (Haldeman) y Bemisia tabaci (Gennadius); mientras que TICV es transmitido únicamente por T. vaporariorum. El rango de huéspedes de estos virus incluye varios cultivos importantes y especies ornamentales. Todas las plantas de tomate procesadas en este trabajo fueron analizadas mediante transcripción reversa de la reacción en cadena de la polimerasa (RT-PCR) utilizando cebadores específicos de ToCV y TICV.
[EN]: During 1997, tomato (Lycopersicon esculentum) crops with symptoms of interveinal yellowing, brozing, brittleness, and rolling of lower leaves were observed in Malaga and Almeria (southern Spain) and Tomato chlorosis virus (ToCV) was detected for the first time in Spain in 2000 as the causal agent of this disease. Later similar symptoms began to be observed in other tomato crops in Sevilla, Murcia, Alicante, Castellón, Mallorca, Tenerife and Gran Canaria and ToCV was also identified as the causal agent in most of the samples analyzed. In additiou, in several tomato samples collected during the summer of 2001 in Castellón province tomato crops a new virus: Tomato infectious chlorosis virus (TICV) was identified. This was the first report of TICV in Spain. To our knowledge, TICV has only been detected in Spain, within the Castellón and Alicante provinces. ToCV and TICV are both whitefly-transmitted criniviruses. They are transmitted in a semipersistent manner and are phloem-limited tomato-infecting viruses. ToCV is transmitted by Trialeurodes vaporariorum (Westwood), T. abutilonea (Haldeman) and Bemisia tabaci (Gennadius); whereas TICV is transmitted only by T. vaporariorum. The host range of these viruses includes some important crops and ornamental species. Reverse transcription polymerase chain reaction (RT-PCR) using ToCV- and TICVspecific primers was used for the reliable diagnosis of ToCV and TICV in field samples
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Trialeurodes abutilonea (Haldeman), Tomato infectious chlorosis virus, mosca blanca, RT-PCR, Tomato chlorosis virus, Bemisia tabaci (Gennadius), Criniviruses, Tomato infect, Whitefly, Tomato, crinivirus, Yellowing, tomate, Tomate, Trialeurodes vaporariorum (Westwood), Lycopersicon esculentum, Mosca blanca, Amarilleo
Trialeurodes abutilonea (Haldeman), Tomato infectious chlorosis virus, mosca blanca, RT-PCR, Tomato chlorosis virus, Bemisia tabaci (Gennadius), Criniviruses, Tomato infect, Whitefly, Tomato, crinivirus, Yellowing, tomate, Tomate, Trialeurodes vaporariorum (Westwood), Lycopersicon esculentum, Mosca blanca, Amarilleo
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