AbstractThe enantiomeric purity of (+/–)‐methyl jasmonate in fresh leaf material of Jasminum from different species and Rosmarinus officinalis was examined by solid‐phase microextraction‐GC‐MS (SPME‐GC‐MS). For comparison with these natural products, commercial jasmine and rosemary fragrances were also studied. The extraction conditions were selected as a result of testing different values of temperature (40, 50, and 60°C) and time (2, 15, 30, and 40 min). The results obtained in this work revealed a range of enantiomeric excesses for (+/–)‐methyl jasmonate varying from 13 to 95% depending on the Jasminum specie considered. In contrast, (–)‐methyl jasmonate always occurred as a pure enantiomer in all R. officinalis samples studied. This implies those Jasminum species in which the enantiomeric purity of (–)‐methyl jasmonate is high enough and any R. officinalis sample might be used as natural sources of pure (–)‐methyl jasmonate. Concerning the commercial fragrances, those of jasmine showed enantiomeric composition of (–)‐methyl jasmonate ranging from 1 to 15% whereas those of rosemary exhibited practically the pure (–)‐methyl jasmonate. This fact suggests the addition and nonaddition of the racemic mixture of methyl jasmonate to the commercial jasmine and rosemary samples, respectively.