
handle: 10261/240958
[ES]: Se estudia la exactitud y reproducibilidad de las técnicas analíticas utilizadas en la extracción y separación de lípidos no absorbidos presentes en las heces de ratas Wistar alimentadas con dietas conteniendo aceites termoxidados. Se ha encontrado una reproducibilidad muy elevada en la cuantificación de lípidos totales, ácidos grasos no polares y composición en ácidos grasos. Por otra parte, la comparación de los lípidos extraídos de las dietas con sus correspondientes aceites de partida, demuestra en primer lugar una total recuperación de la grasa en el proceso de extracción y en segundo ausencia de alteración significativa durante el mismo. Los lípidos no absorbidos se caracterizan por un elevado contenido en ácidos grasos polares y compuestos no saponificables.
[EN]: Reproducibility of the techniques used for isolation and separation of non-absorbed lipids present in feces from rats fed with diets containing heated oils are defined. Highly reproducible results have been found for total lipids, nonpolar fatty acid compositions. Results obtaines from extracted diets demonstrate both the absence of significant alteration and the complete extraction of the fat when compared to those obtained directly from the oils. Non-absorbed lipids from diets containing thermoxidized oils are characterized by high proportions of unsaponifiable compounds and polar fatty acids.
A la Junta de Andalucía y a la Comisión Interministerial de Ciencia y Tecnología, por la financiación aportada.
Peer reviewed
Aceite termoxidado, Dieta, Thermoxidized oil, Rata
Aceite termoxidado, Dieta, Thermoxidized oil, Rata
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