Monitoring the presence and expansion of alien species and upgrading their biological and ecological knowledge seems crucial to mitigate their possible impact on native communities. Within inland superficial waters, alien fish represent an important threat to the biodiversity and studies on their impact on native communities have increased around the world in the last years. However, little is known about their occurrence, biology and influences in North Africa in general, and more specifically in Morocco. In the present work we aimed to: 1) investigate the presence of any native Aphanius species, especially the Mediterranean killifish Aphanius fasciatus recorded from the lower basin of the Moulouya River (NE Morocco); 2) monitor the presence and expansion of two invasive species, the eastern mosquitofish Gambusia holbrooki and the mummichog Fundulus heteroclitus; and 3) contribute to the understanding of the ecological and abiotic affinities that govern the distribution of these alien fishes in North Africa. To achieve these goals, several field sampling campaigns were carried out between 2014 and 2018 across eastern Morocco, comprising the administrative Oriental Region and the Moulouya River Basin and covering an area of 119,268 km2. No native Aphanius species were found. The eastern mosquitofish has invaded the freshwater hydrosystems of the northern part of Morocco, including the study area, while the mummichog is currently limited to the brackish and salty wetlands of Lower Moulouya. Our results show that the known ranges of these two alien species have expanded. Data published through GBIF (Doi:10.15470/2qed9o)