Abstract. Several sets of reference regions have been proposed in the literature for the regional synthesis of observed and model-projected climate change information. A popular example is the set of reference regions introduced in the IPCC Special Report on Managing the Risks of Extreme Events and Disasters to Advance Climate Adaptation (SREX) based on a prior coarser selection and then slightly modified for the 5th Assessment Report of the IPCC. This set was developed for reporting sub-continental observed and projected changes over a reduced number (33) of climatologically consistent regions encompassing a representative number of grid boxes (the typical resolution of the 5th Climate Model Intercomparison Projection, CMIP5, climate models was around 2º). These regions have been used as the basis for several popular spatially aggregated datasets, such as the seasonal mean temperature and precipitation in IPCC regions for CMIP5. Here we present an updated version of the reference regions for the analysis of new observed and simulated datasets (including CMIP6) which offer an opportunity for refinement due to the higher model resolution (around 1º for CMIP6). As a result, the number of regions increased to 43 land plus 12 open ocean, better representing consistent regional climate features. The paper describes the rationale followed for the definition of the new regions and analyses their homogeneity. The regions are defined as polygons and are provided as coordinates and shapefile together with companion R and Python notebooks to illustrate their use in practical problems (trimming data, etc.). We also describe the generation of a new dataset with monthly temperature and precipitation spatially aggregated in the new regions, currently for CMIP5 (for backwards consistency) and CMIP6, to be extended to other datasets in the future (including observations). The use of these reference regions, dataset and code is illustrated through a worked example using scatter diagrams to offer guidance on the likely range of future climate change at the scale of reference regions. The regions, datasets and code (R and Python notebooks) are freely available at the ATLAS GitHub repository; https://github.com/SantanderMetGroup/ATLAS, doi:10.5281/zenodo.3688072 (Iturbide et al., 2020).