Identification of key sequence features required for microRNA biogenesis in plants

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 21 Oct 2020 Argentina, Spain, Spain, Spain, Spain English Publisher:Springer Science and Business Media LLCJournal:Nature Communications, volume 11 (eissn: 2041-1723,

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Authors: Arantxa M. L. Rojas; Salvador I. Drusin; Uciel Chorostecki; Julieta L. Mateos; Belén Moro; Nicolás G. Bologna; Edgardo G. Bresso; +4 Authors

doi: 10.1038/s41467-020-19129-6 , 10.60692/fznzq-jpc60 , 10.60692/4wr31-jr821

pmid: 33087730

pmc: PMC7577975

handle: 2117/345927 , 10261/229170 , 11336/144199 , 20.500.12328/4050

Identification of key sequence features required for microRNA biogenesis in plants

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Abstract

AbstractMicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis.

Countries

Argentina, Spain, Spain, Spain, Spain

Related Organizations

University of Buenos Aires
Argentina
Autonomous University of Barcelona
Spain
National Scientific and Technical Research Council
Argentina
National University of Rosario
Argentina
Instituto de Biología Molecular y Celular de Rosario
Argentina

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Keywords

Biología molecular vegetal, Ribonuclease III, Base Pair Mismatch, Arabidopsis, Cell Cycle Proteins, Plant Science, Gene, Non-coding RNAs, ARNs no codificants, Agricultural and Biological Sciences, Computational biology, RNA interference, https://purl.org/becyt/ford/1.6, Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica, RNA Processing, Post-Transcriptional, Biogenesis, miRNA biogenesis, microRNA, ARNi, Q, Life Sciences, MicroRNA, RNA, Plant, Light Signal Transduction in Plants, :Informàtica::Aplicacions de la informàtica::Bioinformàtica [Àrees temàtiques de la UPC], Cell biology, 5, Ciencias vegetales, Plant molecular biology, Multicellular organism, Science, Arabidopsis., Molecular Dynamics Simulation, Ciències vegetals, Polymorphism, Single Nucleotide, Article, Molecular Mechanisms of Plant Development and Regulation, Drosha, Ribonuclease, Magnoliopsida, ARN no codificantes, Genetics, https://purl.org/becyt/ford/1, Biology, Arabidopsis Proteins, Mutant, Plant Nutrient Uptake and Signaling Pathways, MicroRNAs, RNAi, FOS: Biological sciences, Biologia molecular vegetal, Nucleic Acid Conformation, RNA, Molecular Dynamics simulations, Gene expression, Plant sciences, Genètica, Dicer

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