Triclosan (TCS) has been immensely employed in health care products and consumer items, as an active agent with fungicidal and bactericidal potentialities, such as soaps, sanitizers, tubes of toothpaste, deodorants, skin creams, and so on for over last five decades. The ultimate excretory route of TCS ends in our water matrices, thus has been frequently detected with ecological and human-health related matters and hazards. Bioactive residues of TCS reach into the key atmosphere compartment through numerous routes, such as (1) scarce or ineffective elimination or degradation throughout the treatment practices, (2) abandoned landfill leachates, (3) leakage from the discarded TCS-containing materials, and so on. Such persistence and occurrence of TCS or its degraded but bioactive residues have growing attentions. Its complete removal and/or effective prevention are still challenging tasks for safeguarding the environment. Owing to the highly effective catalytic and stability potential, enzyme-based bio-degradation approaches are considered an evocative substitute for TCS mitigation from environmental matrices. As compared to enzymes in their pristine form, immobilized enzymes, with unique catalytic, stability, selectivity, and reusability profile, are of supreme and strategic interest in environmental biotechnology. Herein, an effort has been made to signify the novel bio-catalytic and bio-degradation potentialities of various oxidoreductases, including laccases, and peroxidases including soybean peroxidase, versatile manganese peroxidase, and horseradish peroxidase with suitable examples. Following a brief introduction, the focus is given to the presence of TCS in the key atmosphere compartments. Potential sources, acquaintance, and hazardous influence of TCS are also discussed with recent and relevant examples. The second half shows the TCS removal/degradation potentialities of soluble enzyme-based catalytic systems and immobilized-enzyme-based catalytic systems. Finally, the concluding remarks, along with possible future directions are given in this significant research arena.