The presence of pesticides in agricultural wastewater entails harmful risks to both the environment and public health. In this study, two channel-type bioreactors with Trametes versicolor immobilized on pinewood chips were evaluated in terms of the removal efficiency of diuron from agricultural wastewater under non-sterile conditions. First, both single and successive sorption processes of diuron on pinewood chips were evaluated. The Freundlich model showed the best correlation in the sorption isotherm study (R2 = 0.993; Δq = 5.245), but according to repeated sorption experiments, the Langmuir model (R2 = 0.993; Δq = 5.757) was considered more representative. Equilibrium was reached after approximately 48 h, and the Elovich kinetic model gave the best fit with the experimental data. A packed-bed channel bioreactor (PBCB) was found to be a remarkable alternative able to remove up to 94% diuron from agricultural wastewater during 35 d. However, periodic manual mixing was required to guarantee an aerobic process, and a rotating drum bioreactor (RDB) was subsequently proposed as an enhanced version. The RDB removed up to 61% diuron during 16 d using almost 7 times lower wood dose (152 g wood·L-1) than in the PBCB (1000 g wood·L-1).