A selective culture medium for screening linezolid-resistant gram-positive bacteria

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 2019 Spain, Switzerland English Publisher:Elsevier BVJournal:Diagnostic Microbiology and Infectious Disease, volume 95, pages 1-4 (issn: 0732-8893,

Copyright policy )

Authors: Nordmann, Patrice; Rodríguez-Villodres, Angel; Poirel, Laurent;

doi: 10.1016/j.diagmicrobio.2019.03.006

pmid: 30981556

handle: 10261/213136 , 10668/13824

A selective culture medium for screening linezolid-resistant gram-positive bacteria

- Summary
- Subjects
- Metrics

Abstract

The SuperLinezolid medium was developed for screening resistance to linezolid (LZD) in Gram-positive bacteria (Staphylococcus spp., Enterococcus spp.). It was evaluated using LZD-susceptible (n = 20) and LZD-resistant (n = 17) Gram-positive isolates. The sensitivity was found to be 82% at 24 h (3 out of 17 isolates being missed), and reached 100% at 48 h. At 48 h, a single LZD-susceptible isolate grew (specificity 95%). By testing stools spiked with LZD-resistant Gram-positive strains, an excellent performance of the medium was observed, with a lowest detection limit ranging from 101 to 102 CFU/ml. Overall, this medium is accurate for detection of LZD-resistant Gram-positive isolates after 24 h of culture.

Countries

Spain, Switzerland

Related Organizations

Spanish National Research Council
Spain
University of Seville
Spain
University of Lausanne
Switzerland
Hospital Universitario Sanitas Virgen del Mar
Spain
Centre Hospitalier Universitaire de Reims
France

View all View all

Keywords

Bacteriological Techniques, Enterococci, Staphylococcus, info:eu-repo/classification/udc/57, Antibiotic, Linezolid, Microbial Sensitivity Tests, Gram-Positive Bacteria, Sensitivity and Specificity, Anti-Bacterial Agents, Culture Media, Feces, Antibiotics, Drug Resistance, Bacterial, Screening, Humans, Enterococcus, Staphylococci

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	6
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%