A portable epigenetic switch for bistable gene expression in bacteria

descriptionPublicationkeyboard_double_arrow_right Article 02 Aug 2019 Spain, Sweden English Publisher:Springer Science and Business Media LLCJournal:Scientific Reports, volume 9 (eissn: 2045-2322,

Copyright policy )Funded by:VR | unidentified

Authors: David R. Olivenza; Hervé Nicoloff; María Antonia Sánchez-Romero; Ignacio Cota; Dan I. Andersson; Josep Casadesús;

doi: 10.1038/s41598-019-47650-2

pmid: 31375711

pmc: PMC6677893

handle: 11441/89064 , 10261/207037

A portable epigenetic switch for bistable gene expression in bacteria

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Abstract

AbstractWe describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.

Countries

Spain, Sweden

Related Organizations

Spanish National Research Council
Spain
Center for Research in Agricultural Genomics
Spain
Autonomous University of Barcelona
Spain
University of Seville
Spain
Uppsala University
Sweden

Keywords

Salmonella typhimurium, Science, Biosensing Techniques, Biochemistry, Article, Epigenesis, Genetic, Disk Diffusion Antimicrobial Tests, Drug Resistance, Bacterial, Operon, Escherichia coli, Promoter Regions, Genetic, Biokemi, Molecular Biology, Molekylärbiologi, DNA methylation, Q, Biochemistry and Molecular Biology, R, Gene Expression Regulation, Bacterial, DNA Methylation, Anti-Bacterial Agents, Bacterial synthetic biology, Genes, Bacterial, Mutation, Mutagenesis, Site-Directed, Medicine, Synthetic Biology, Biokemi och molekylärbiologi, Plasmids

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