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Somatic Embryogenesis from Roots of Camellia japonica Plantlets Cultured in Vitro

Authors: Viéitez Martín, Ana María; San José, M. Carmen; Viéitez Madriñán, Francisco Javier; Ballester, Antonio;

Somatic Embryogenesis from Roots of Camellia japonica Plantlets Cultured in Vitro

Abstract

Somatic embryos were induced on the roots of Camellia japonica L. plantlets regenerated from an in vitro clone of juvenile origin. The embryos appeared to differentiate from epidermic cells and to be connected with the root via a few parenchymatous cells. Somatic embryogenesis occurred on basal medium and with or without various combinations of zeatin, BA, and IBA. Secondary embryos were induced on cotyledons and/or hypocotyl regions of somatic embryos. Two morphological types of somatic embryos were developed, seed-like and bud-like types, and their formation was influenced by the presence of BA in the medium. Embryogenic capacity has been maintained for more than 24 months by subculturing secondary embryos at 7- to 8-week intervals. The best gibberellin/auxin combination for inducing the germination of isolated somatic embryos was GA at 5 mg·liter -1 G A 3 and IAA at 1 mg·liter -1 . P1antlets were successfully established in planting medium and have continued to grow in a greenhouse. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA); (1α, 2β, 4aα, 4bβ, 10β)-2,4a,7-trihydroxy-l-methyl-8-methylenegibb-3-ene-1,10-dicarboxylic acid l,4a-lactone (GA); 1 H -indole-3-acetic acid (IAA); 1 H- indole-3-butyric acid (IBA); 2-methyl-4-(1 H- purine-6-ylamino)-2-buten-l-ol (zeatin).

Related Organizations
Keywords

Camellia, Micropropagation, Plant tissue culture, Secondary embryogenesis

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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