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Molecular ontogeny of digestive enzymes in Persian sturgeon (Acipenser persicus)

Authors: Gilannejad, Neda; Heyrati, Fatemeh Paykan; Dorafshan, Salar; Martos-Sitcha, Juan Antonio; Yúfera, Manuel; Martínez-Rodríguez, Gonzalo;

Molecular ontogeny of digestive enzymes in Persian sturgeon (Acipenser persicus)

Abstract

The Persian sturgeon (Acipenser persicus) is a species of primary commercial interest which inhabits the Caspian and Black seas. This species is overfished mainly because of the high value of its roe and, in consequence, is listed as a critically endangered species. Sea ranching and production in hatchery is envisioned as a way to solve the problem. It is therefore necessary to advance the knowledge of the biology of larvae and juveniles to improve the rearing techniques. During the early development of the digestive system, digestive capacity is provided by the pancreatic enzymes (proteases, lipases and glucosidases) in conjunction with alkaline proteolytic enzymes secreted by the intestine, prior to acid digestion. While the pattern of digestive activities during the ontogeny has been profusely studied in many species, the expression patterns of the mRNA transcripts encoding the digestive enzyme precursors are comparatively scarce (Rønnestad et al., 2013). In this study, cDNAs coding for trypsinogen (try), two isoforms of pepsinogen (pga), proton pump (atp4a), bile salt activated lipase (cel) and β-actin (actb) from Persian sturgeon were partially cloned and their gene expression were analyzed using real time PCR from hatching to 34 days post hatch (dph).

The present work has been partially financed by the Center for International Scientific Studies & Collaboration (CISSC), Iranian Ministry of Science, Research and Technology

Trabajo presentado en el Aquaculture Europe 16 (Food for Thought), celebrado en Edimburgo del 20 al 23 de septiembre de 2016.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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