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handle: 10261/166142
Grapevine is a traditional and the most important crop for Armenian agriculture. Nematodes of the genus Xiphinema are migratory root ectoparasites that feed mainly on the root-tips of a wide range of wild and cultivated plant species. Several species also transmit nepoviruses. There is few information regarding the occurrence and distribution of Xiphinema species in theRepublic ofArmenia, especially virus vector species. The data concerning fauna of plant parasitic nematodes of Nagorno Karabakh region is absent. The aim of this research was to analyze the occurrence of Xiphinema species in vineyards of the Republic of Armenia and region of Nagorno Karabakh. For that, plants and soil samples were collected from four mountain regions (Tavush, Vayots-Dzor, Aragatsotn, Kotayk) and two regions of Ararat valley (Armavir, Ararat) of the Republic of Armenia and two regions of Nagorno Karabakh (Martuny and Askeran). Soil samples including fine roots in the rhizosphere of Vitis vinifera “Areni”, “Ararati”, “Mskhali”, “Rkatsiteli”, “Voskehat” varieties with symptoms Grapevine fan leaf virus (GFLV) infection were collected. Morphological and molecular identification of the isolated species was carried out. Morphological measurements were performed on temporary and permanent glycerin slides. Molecular identification was carried out b y using the D2-D3 expansion segments of the 28S rRNA gene and the ITS region of individual nematodes from Vayots-Dzor, Aragat sotn regions and Ararat valley.
Migunova, V. et al.-- Trabajo presentado en el XVIII International Plant Protection Congress (Mission Possible: food for all throught appropiate plant protection), celebrado en Cuba del 11 al 15 de mayo de 2015.
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