The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

descriptionPublicationkeyboard_double_arrow_right Article 05 Jun 2017 Spain English Publisher:Springer Science and Business Media LLCJournal:Scientific Reports, volume 7 (eissn: 2045-2322,

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Authors: B. Mir; X. Solés; C. González; N. Escaja;

APC: 978.75 EUR

doi: 10.1038/s41598-017-02723-y

pmid: 28584239

pmc: PMC5459817

handle: 2445/112375 , 10261/165896

The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

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Abstract

AbstractIncorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C+ stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C+ or psC:psC+ hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.

Country

Spain

Related Organizations

University of Barcelona
Spain
Spanish National Research Council
Spain
Institute of Physical Chemistry "Rocasolano"
Spain

Keywords

Models, Molecular, Magnetic Resonance Spectroscopy, Science, Q, Àcids nucleics, R, Oligonucleotides, Oligonucleòtids, Cytidine, Hydrogen-Ion Concentration, Article, Nucleic acids, Medicine, Nucleic Acid Conformation, Thermodynamics, Citidina difosfat de colina, Nucleotide Motifs, Protons, Cytidine diphosphate choline

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