
handle: 10261/16504
[ES] En el presente trabajo se comparan los resultados obtenidos en la cuantificación de pigmentos fotosintéticos en hoja de remolacha azucarera, según las fórmulas recomendadas por diferentes autores y mediante el análisis por HPLC. Asímismo se hace un estudio de tres procedimientos de extracción y se comparan varios solventes orgánicos con extractantes, demostrando que, de todos ellos, el más eficaz es la acetona del 100%. Se pone de manifiesto que la relación entre las clorofilas a y b dependen del solvente utilizado y de la ecuación aplicada en el cálculo de las mismas.
[EN] In this paper, sugar beet photosynthetic pigments, as determinated quantitatively by HPLC, are discussed in relation to those obtained using several equations. Three extraction methods are also compared. The 100% acetone has been the most efficient solvent among the various organic reagents tested. On the other hand, it seems that the chlorophyll a/b ratio depends on the extractant and the equation used.
Trabajo financiado por la CAICYT (Proyecto 6142-02).
Peer reviewed
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