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Análisis cuantitativo de pigmentos en plantas superiores por HPLC

Authors: Monge Pacheco, Emilio; Val Falcón, Jesús; Heras Cobo, Luis;

Análisis cuantitativo de pigmentos en plantas superiores por HPLC

Abstract

[ES] En el presente trabajo se comparan los resultados obtenidos en la cuantificación de pigmentos fotosintéticos en hoja de remolacha azucarera, según las fórmulas recomendadas por diferentes autores y mediante el análisis por HPLC. Asímismo se hace un estudio de tres procedimientos de extracción y se comparan varios solventes orgánicos con extractantes, demostrando que, de todos ellos, el más eficaz es la acetona del 100%. Se pone de manifiesto que la relación entre las clorofilas a y b dependen del solvente utilizado y de la ecuación aplicada en el cálculo de las mismas.

[EN] In this paper, sugar beet photosynthetic pigments, as determinated quantitatively by HPLC, are discussed in relation to those obtained using several equations. Three extraction methods are also compared. The 100% acetone has been the most efficient solvent among the various organic reagents tested. On the other hand, it seems that the chlorophyll a/b ratio depends on the extractant and the equation used.

Trabajo financiado por la CAICYT (Proyecto 6142-02).

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green