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Variabilidad en poblaciones de Brassica napus españolas e inglesas determinada por marcadores RAPDs

Authors: Cartea González, María Elena; Padilla, Guillermo; Soengas, Pilar; Ordás Pérez, Amando;

Variabilidad en poblaciones de Brassica napus españolas e inglesas determinada por marcadores RAPDs

Abstract

[EN] The genetic diversity and the relationships among a collection of Brassíca napus L. European populations were evaluated using random amplified polymorphic DNA (RAPDs) markers. The study included 33 accessions collected from Galicia (northwestern Spain) and 18 British cultivars. DNA from twenty-five individuals per population was ana!yzed using 18 decamer primers. One hundred thirty-eight amplification products were scored of which 105 were po!ymorphic. Eight groups were obtained. Cluster I was the largest and included all the !andraces from northwestern Spain, except two accessions that grouped separately into clusters III and IV, respectively. A low level of genetic variability was detected among the Spanish genotypes, while considerable diversity was present among the British ones, which grouped into five groups. Cluster II included all commercial varieties grown in Great Britain whereas Cluster V grouped local varieties maintained by growers for many years. Cluster VI, VII, and VIII were singularities forrned by one entry. Two British accessions had the greatest dissimilitud with all the other populations. As conclusion, B. napus landraces used in northwestem Spain as leafy-green vegetable probably have an independent origin from B. napus crops grown in other European regions. Besides, separate domestication in northwestern Spain and Great Britain for a different end use might have led to two distinct gene pools.

[ES] El objetivo de este trabajo es evaluar la diversidad genética y las relaciones entre 33 poblaciones gallegas y 18 poblaciones inglesas de Brassica napus L. mediante RAPDs. Se encontraron 138 bandas RAPDs de las cuales 105 fueron polimórficas. Las 51 poblaciones se agruparon en ocho grupos. El grupo I fue el mayor e incluyó todas las poblaciones locales del Noroeste de España, excepto dos entradas que se agruparon solas en los grupos III y IV, respectivamente. Se encontró un bajo nivel de variabilidad genética entre las poblaciones españolas mientras que entre las inglesas se halló una considerable diversidad. El grupo II incluyó todas las variedades comerciales cultivadas en Gran Bretaña mientras que el grupo V agrupó variedades locales mantenidas tradicionalmente por los agricultores. Los grupos VI, VII, y VIII estuvieron formados por una sola población. Las variedades locales de B. napus utilizadas en Galicia tienen probablemente un origen independiente de las formas de B. napus cultivadas en otras regiones de Europa. La domesticación independiente en el Noroeste de España y en Gran Bretaña para diferentes usos podría haber conducido a una divergencia genética entre los dos grupos.

Investigación financiada por la Excma. Diputación Provincial de Pontevedra, España. P. Soengas agradece la beca de La Xunta de Galicia y G. Padilla agradece la beca del Cabildo Insular de la Palma.

Comunicación presentada en el Congreso de Mejora genética de plantas, celebrado en Almería entre el 25 y el 27 de septiembre de 2002.- 7 páginas y 1 figura.

Peer reviewed

Country
Spain
Related Organizations
Keywords

Taxonomía numérica, Brassica napus, Molecular markers, Diversidad genética, Marcadores moleculares, Genetic diversity, Numerical taxonomy

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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