Molecular basis of quinolone resistance in Escherichia coli from wild birds.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2004 English Publisher:Canadian Veterinary Medical AssociationJournal:Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, volume 68 (issn: 0830-9000,

Copyright policy )

Authors: Jiménez Gómez, P. A.; García de los Ríos, José Esteban; Rojas Mendoza, Ana M.; Pedro Ramonet, P. de; García Albiach, R.; Reche Sainz, M. P.;

pmid: 15352551

pmc: PMC1142146

handle: 10261/153712

Molecular basis of quinolone resistance in Escherichia coli from wild birds.

- Summary
- Subjects
- Metrics

Abstract

Nine quinolone resistant (minimal inhibitory concentration [MIC] was > 32 microg/mL for nalidixic acid, > 1 microg/mL for ciprofloxacin) isolates of Escherichia coli have been found in wild birds with septicemia. All of the isolates were aerobactin positive. The mechanisms of resistance were characterised by sequencing the quinolone resistance-determining region (QRDR) of the gyrA, gyrB, parC, and parE genes. Sequence analysis of the gyrA gene in all isolates identified only 1 nucleotide substitution at codon Serine-83 for Leucine-83. Sequence analysis of the gyrB, parC, and parE QRDR genes revealed no mutations in any of the isolates. This study was conducted to determine the importance of these genes in the susceptibility of E. coli strains isolated from wild birds to quinolones.

This work has been supported by grant 13/99 of the Universidad San Pablo C.E.U.

Peer reviewed

Related Organizations

CEU San Pablo University
Spain

Keywords

Bird Diseases, Animals, Wild, Microbial Sensitivity Tests, Polymerase Chain Reaction, Birds, Anti-Infective Agents, Genes, Bacterial, Sepsis, Drug Resistance, Bacterial, Mutation, Escherichia coli, Animals, DNA Primers, Fluoroquinolones

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	5
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average