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DIGITAL.CSIC
Article . 2017 . Peer-reviewed
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Utilisation d'un outil sérologique pour estimer l'exposition des Sangliers à M. Bovis

Authors: Richomme, Céline; Boadella, Mariana;

Utilisation d'un outil sérologique pour estimer l'exposition des Sangliers à M. Bovis

Abstract

[FR]: Le sanglier (Sus scrofa) est de plus en plus considéré comme un acteur important à prendre en compte dans l'épidémiologie de la tuberculose bovine (TB); le suivi de la TB chez cette espèce revêt alors un enjeu important pour établir des programmes globaux de lutte contre cette maladie. Afin d’estimer en France l'exposition des sangliers sauvages au complexe Mycobacterium tuberculosis (MTC), un ELISA fondé sur un dérivé de protéine bovine purifiée (PPD) a été utilisé pour tester une sérothèque de 2 080 échantillons provenant d’animaux prélevés à la chasse dans 58 départements entre 2000 et 2010. Deux valeurs seuils ont été utilisées pour l'interprétation diagnostique : le seuil de 0,2, recommandé par le fabricant (spécificité: 96,4 %; sensibilité: 72,6%) et le seuil de 0,5 (spécificité: 100%; sensibilité: 65%). Une bonne cohérence spatio‐temporelle entre les sangliers séropositifs et les foyers de TB chez les bovins est mise en évidence, en particulier à la valeur seuil de 0,5 (la distance moyenne entre un foyer bovin et les sangliers séropositifs les plus proches étant de 13 km). Au delà de permettre une première description de la séroprévalence globale et de la répartition géographique de l’exposition au MTC des sangliers en France, nos résultats suggèrent que le test ELISA peut être utilisé à une échelle populationnelle comme un premier outil de surveillance de la TB chez les sangliers, suivi d’éventuelles investigations complémentaires combinant examens lésionnels et culture.

[EN]: The Eurasian wild boar (Sus scrofa) is increasingly considered as a significant actor in the epidemiology of animal tuberculosis (TB). Therefore, monitoring TB in wild boar becomes a key tool in the development of comprehensive control schemes for this disease. To estimate the exposure of free living wild boars to the Mycobacterium tuberculosis complex (MTC) in France, a bovine‐purified protein derivative (PPD) based ELISA was used to test 2,080 archived serum samples from hunter‐harvested animals in 58 French “départements”. Two cut‐off values were used for diagnostic interpretation: 0.2, recommended by the manufacturer (specificity: 96.4%; sensitivity: 72.6%), and 0.5 (specificity: 100%; sensitivity: 64%). A good consistence between seropositive wild boar and TB outbreaks in cattle was found, especially at the 0.5 cut‐off value (the mean distance to the nearest cattle TB outbreak was 13km for seropositive wild boars). Our results provide a first description of the global seroprevalence and geographic distribution of MTC contact in wild boars in France but they also suggest that the ELISA could be used as a first screening tool to conduct TB surveillance in wild boars at a population level, to be completed, in case of seroposivity, by investigations combining pathology and culture.

L’IREC remercie enfin le projet européen Aphaea (EMIDA ERA‐NET).

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et al.

Country
Spain
Related Organizations
Keywords

Epidemiology, Sus scrofa, Tuberculose bovine, Wildlife, Wild boar, Mycobacterium bovis, Faune sauvage, Bovine tuberculosis, Sérologie, Épidémiologie, Zoonosis, Serology, Zoonose, Sanglier, Complexe Mycobacterium tuberculosis, Mycobaterium tuberculosis complex

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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