A COMPARATIVE STUDY OF SUPPORTS FOR THE SYNTHESIS OF OLIGONUCLEOTIDES WITHOUT USING AMMONIA

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1996 Spain English Publisher:Informa UK LimitedJournal:Nucleosides and Nucleotides, volume 15, pages 1,871-1,889 (issn: 0732-8311,

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Authors: Aviñó, Anna; Güimil García, Ramón; Díaz, Antonio R.; Albericio, Fernando; Eritja Casadellà, Ramón;

doi: 10.1080/07328319608002739

handle: 10261/141171

A COMPARATIVE STUDY OF SUPPORTS FOR THE SYNTHESIS OF OLIGONUCLEOTIDES WITHOUT USING AMMONIA

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Abstract

A comparative study of the cleavage efficiency of succinyl, phthaloyl, oxalyl, 2(2-nitrophenyl)ethyl, 9-fluorenylmethyl, and 2-nitrobenzyl supports in 0.5M DBU solutions is described. A decrease in cleavage efficiency is observed when small oligonucleotides containing thymidine are linked to the supports. In these conditions oxalyl supports gave the best yields followed by 2-(2-nitrophenyl)ethyl and 9-fluorenylmethyl supports.

We are grateful to CICYT (PB92-0043) and E.E.C.C. Biomedicine and Health Programme (BMH1-CT93-1669) for financial support. We thank Drs. Matthias Mann, Gitte Neubauer, Matthias Wilm (EMBL) and Irene Fernández (University of Barcelona) for obtaining mass spectra.

Peer reviewed

Country

Spain

Related Organizations

Spanish National Research Council
Spain
European Molecular Biology Laboratory
Germany
University of Barcelona
Spain
Centro de Investigación y Desarrollo
Spain
European Molecular Biology Laboratory
Germany

Keywords

Drug synthesis, Structure activity relation, Oligonucleotides, Nucleotide derivative

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