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handle: 10261/125985
[ES] Se presentan dos procedimientos histológicos que permiten la inclusión de las plumas en parafina, con el fin de obtener secciones apropiadas para su uso en microscopía óptica. Las técnicas se basan en el tratamiento (1) con yoduro potásico y (2) con ferricianuro férrico. En ambos casos se posibilita el corte de la pluma, permitiendo la observación de su estructura interna (p.e. distribución de melanosomas) y la aplicación de protocolos adicionales, como tinciones convencionales o técnicas de inmunohistología.
[EN] We present two histological procedures that allow the paraffin embedding of feathers in order to get appropriate sections for their use in optical microscopy. Technics are based in treatment (1) with potassium iodide and (2) with ferric ferricyanide. In both cases the cut of feather is enabled, allowing the observation of its internal structure (e.g. melanosomes distribution) and the use of additional protocols as traditional staining or immunohistology techniques.
Peer Reviewed
Queratina, Disulfide bond, Melanosoma, Keratin, Feather, Pluma, Puente disulfuro, Melanosome
Queratina, Disulfide bond, Melanosoma, Keratin, Feather, Pluma, Puente disulfuro, Melanosome
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