© 2015 Elsevier Ltd. An anti-inflammatory peptide was identified in lupine protein hydrolysate (LPH) obtained by enzymatic hydrolysis of lupine protein isolate (LPI) with Alcalase. LPH was separated in two fractions by ultrafiltration, and anti-inflammatory activity was evaluated by analysis of the expression of different cytokines in THP-1-derived macrophages by quantitative PCR (qPCR). Fraction <10 kDa was purified by chromatographic techniques, and the most active subfraction was analysed by mass spectrometry. A novel anti-inflammatory peptide was identified and the sequence was Gly-Pro-Glu-Thr-Ala-Phe-Leu-Arg. This peptide was synthesised, and its anti-inflammatory activity was tested at two concentrations. Expression of TNF, IL-1β, and CCL2 was significantly reduced whereas the expression of the anti-inflammatory cytokine IL-10 was induced. Regarding the production of cytokines, only the lowest concentration of the synthetic peptide showed anti-inflammatory properties. Furthermore, the synthetic peptide decreased nitric oxide production. Thus, the identified peptide may help to prevent chronic inflammation associated with several diseases.

This work was supported by grant AGL2012-40247-C02-01 from the Spanish Ministry of Economy and Competitiveness.

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